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6 years ago in Case Study , Discussion By Keerthi Gupta

Steps involved in replica plating technique

What is the basic principle behind replica plating technique? How is it done? Any answers? Thank you in advance.

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All Answers (4 Answers In All)

By Vipin Kumar Answered 6 years ago

This method is used to detect biochemical mutants for the determination of the spectra of antibiotic sensitivity and for the classification of fermentation reactions. The principle used here is, threads of velvet or chamois leather are utilised( acting as inoculating needles) the mutants can be replicated and isolated.

By Zeba Malik Answered 6 years ago

The replica plating procedure is very simple.
1. Place a piece of sterile velvet on a cylindrical metallic block .
2. Mount the block with velvet facing upwards.
3. Invert the petri dish that includes the lawn of bacterial cells and press against the velvet. The projection fibres of the velvet act as inoculating needles and samples every single clone of the cells in the lawn. Next remove the petri dish and press dual phage coated agar against the velvet and save the original master plate.
5. This is followed by incubating the subsequent phage coated plates.
6. Colonies that appear on the phage coated plates represents mutant that arose during the occurrence of the cell divisions.
7. Mark the position and pick up the colony from the same location from the master plate.
9. Put this to a tube of broth and incubate them to increase the total cell number and spread the sample over a fresh agar plate.

By Yashti Iyer Answered 6 years ago

Keerthi, Zeba has explained the procedure. But if you want to know more about this technique watch https://www.youtube.com/watch?v=exWgCRn0d84, https://www.youtube.com/watch?v=uYb-_hYSVYw.

By Keerthi Gupta Answered 6 years ago

Thank you so much for the explanation and the links guys.

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