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4 years ago in PCR , Polymerase Chain Reaction , Serum By Anoush
Is it correct to diagnose HCV through plasma in the RT PCR test?
 I am conducting research and for that I have to diagnose Hepatitis C Virus. I considered using the RT PCR test but now I am confused whether I should use serum or plasma. Some say serum is best but others say plasma is more valid.Â
What should I do? Any opinions please.
All Answers (4 Answers In All)
By Sato Answered 4 years ago
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By Henry Answered 4 years ago
So, as Sato said above, you can choose any of the two options.
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By Kirti Answered 4 years ago
Hello dear, as the experts above have already mentioned, plasma and serum both can be effectively used to detect the HCV RNA and can also be used to quantify them. However, if you still need a detailed answer as to how you can make your choice, I am attaching a few links to similar articles based on this. I hope you get your confusion cleared and do well in your research. Â Thank you and good luck. Â
https://link.springer.com/protocol/10.1007%2F978-1-59745-394-3_1
https://www.wjgnet.com/1007-9327/full/v3/i2/108.htm
https://journals.asm.org/doi/10.1128/JCM.37.3.484-489.1999
https://linkinghub.elsevier.com/retrieve/pii/S0166093402000071
https://onlinelibrary.wiley.com/doi/10.1002/jmv.1890470208
Replied 4 years ago
By Anoush
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By Anoush Answered 4 years ago
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