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Seeking recommendation for N-terminal specific Anti-Histidine Antibody

9 months ago in Biology , Chemistry , PhD Interview Question By Reema

PhD Interview Scientific Research

what factors should be considered when selecting an appropriate antibody for this application

Can anyone suggest a reliable N-terminal specific Anti-Histidine Antibody for use in protein detection assays, such as Western blotting or immunohistochemistry, and what factors should be considered when selecting an appropriate antibody for this application

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By Sur M Answered 9 months ago

When searching for an N-terminal specific Anti-Histidine Antibody, it's crucial to consider factors such as antibody specificity, sensitivity, and compatibility with the intended application. Several commercial suppliers offer antibodies specifically designed to target the N-terminal region of proteins containing Histidine tags, commonly used in recombinant protein expression systems. Additionally, researchers may explore peer-reviewed literature or consult with colleagues to identify validated antibodies with demonstrated performance in detecting Histidine-tagged proteins. Prioritize antibodies with proven specificity for the N-terminal region and optimize assay conditions to maximize detection sensitivity and minimize non-specific binding

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