Post Your Answer
Southern blotting method vs Northern blotting for identifying target DNA
5 years ago in Data Analysis By Pooja
All Answers (3 Answers In All)
By Shashank Answered 4 years ago
northern blotting the extension of the southern blotting method. However, northern blotting method is mostly used to identify RNA and not DNA. you have to use southern blotting method for DNA fragments identification.
By Shraddha Answered 4 years ago
sure.The basic principle behind southern blotting method is: in this technique the property of a radio-labelled probe is exploited using a single stranded DNA. however, If you want to determine the presence of a particular sequence in mixed DNA sample then the probe must be designed accordingly which have complementary sequence to the target sequence. To know more about this technique visit https://www.khanacademy.org/test-prep/mcat/biomolecules/dna-technology/v/southern-blot.
By Trisha Answered 4 years ago
In southern blotting method, DNA from the sample must be cleaved into restriction fragments using a restriction endonuclease. These fragments are then spread apart via gel electrophoresis. The double-stranded helix of the DNA fragment must be denatured into single strands using the pH of gel basic. The is then blotted with nitrocellulose, thereby transferring few DNA strands to the nitrocellulose sheet. Next, a probe including purified, single-stranded DNA relevant to specific gene is transformed into the sheet. The DNA fragment that has a nucleotide sequence will hybridise with the probe. If the probe if is 32P, will be radioactive, and the nitrocellulose sheet will display a band of radioactivity where the probe will be hybridised with fragment specific gene.
asked at 30 Jul, 2022 13:35 in Data Analysis By Anu
asked at 30 Aug, 2021 10:56 in Data Analysis By Aarushi Duneja
asked at 29 Aug, 2021 05:41 in Data Analysis By Venu M
asked at 26 Aug, 2021 04:36 in Data Analysis By Jhanvi Saluja
asked at 09 Jan, 2020 10:23 in Data Analysis By Raghu
asked at 09 Jan, 2020 10:10 in Data Analysis By Akash
asked at 01 Jan, 2020 18:15 in Data Analysis By Natasha
asked at 30 Nov, 2019 04:05 in Data Analysis By Bindya
asked at 21 Nov, 2019 11:25 in Data Analysis By Adi
asked at 12 Nov, 2019 03:04 in Data Analysis By Itjarz
asked at 06 Nov, 2019 02:34 in Data Analysis By Komal
asked at 04 Nov, 2019 01:34 in Data Analysis By Umar Nasiru
asked at 23 Oct, 2019 12:45 in Data Analysis By Nazia
asked at 22 Oct, 2019 14:18 in Data Analysis By Pragati
asked at 18 Oct, 2019 15:37 in Data Analysis By Natasha
717122 Bhagirathi Bisht
Query regarding image processing
653563 Sonam Bhatia
749713 Muhammad Umar Farooq
Disagreements between my supervisor and me
773344 Anju Mehera
716442 Muhammad Umar Farooq
Difference between multivariate and bivariate analysis
848412 Lalit Mudra
697371 Rahul Kohli
710337 Raghav V
Peer reviews are reliable or not?
678515 Priyanshu Rathore
- Angular
- Research Objectives
- PhD Admissions
- Action Research
- APA Style
- Annexure I Journals
- Academic Writing
- Abstract
- Architecture
- Architecture
- Assignments
- Bibliography
- Case Study
- Citations
- Concept Matrix
- Concept Paper
- Conceptual Framework
- Conclusion
- Content Analysis
- Corrections
- Cross Sectional Study
- CST Software
- PhD Data
- Data analysis
- Data Collection
- Data Analysis
- Descriptive Statistics
- Design
- Discussion
- Dissertation
- Draft
- Editing
- Empirical Paper
- Engineering
- English literature
- Ethnobotanical
- Ethnographic Method
- Excel
- Executive Summary
- Financial analysis
- Formatting
- Grammarly
- Grounded theory
- Guidelines
- HR
- Hypothesis
- Impact Factor Journals
- Interview
- Introduction
- Java
- Journal
- LabVIEW
- Latex
- Literary Analysis Techniques
- Literature
- Literature Review
- Longitudinal study
- Management
- Material for study
- Matlab
- Methodology
- MLA Format
- MLA Style
- Objectives
- Peer Review
- Paper Publication
- PhD
- PhD Funding
- PhD Interview
- PhD planner
- PhD Thesis
- PhD Management
- Pilot Study
- Plagiarism Check
- Presentation
- Psychology
- Qualitative Data
- Quantitative methods
- Qualitative Method
- Qualitative research
- Qualitative Research
- Quantitative research
- Questionnaire
- References
- Referencing
- Report Writing
- Research design
- Research Methodology
- Research methods
- Research objective
- Research Paper
- Research philosophy
- Research Problem
- Research Proposal
- Research Question
- Research Hypotheses
- Review Paper
- Revisions
- Sample
- SCI Journals
- Secondary Data Analysis
- Secondary Source
- Software
- Software for Plagiarism
- SPSS
- SQL
- SSCI Journals
- STATA
- Statistical Tests
- Structural Analysis
- SWOT Analysis
- Synopsis
- Technical Writing
- Thematic Analysis
- Thomson Reuters
- Topic
- Topic Selection
- Turnitin
- University Guidelines
- Variables
- Writing
- Writing Editing
- Testtag
- Dissertation
- Simulation
- Coding
- Scientific Manuscript
- Algorithms
- Design
- Software
- Statistical Analysis
- Analysis
- Supervising
- Parametric Test
- Parameter
- Submission
- Base Paper
- Interpretation
- Dissipation Systems
- Data Science
- Machine Learning
- Hybrid Electric
- Power Control
- ArcGiS
- Spatial Analysis
- Switching
- Simulink
- Artificial Intelligence
- Deep Learning
- Panel Data
- Reliability
- Pandemic
- COVID-19
- HRM
- Ansys
- Multiphase Flow Modelling
- Remote Sensing Software
- ENVI
- Qualitative Research
- Thinking
- Likert Scale
- Scale Construction
- Sample Size
- Methodology
- Questionnaire
- Regression
- Linear Equation
- Linear Programming
- Wireless Communications
- Digital Communications
- Wireless Network
- Publications
- Publications
- Scientific Research
- Convergent Variant
- Conferences
- Conferences
- Abstracts
- Bioinformatics
- Differential Gene
- Survey
- Somatic Cell Nuclear Transfer
- Research Design
- Writing
- Microsoft Windows
- Student
- Circuits
- Digital
- Serum
- Plasma
- Polymerase Chain Reaction
- Solar Collector
- Heat Transfer
- Radiation
- API
- Python
- Research Paper
- Design Thinking
- Training
- Psychology
- Python
- R Programming
- Primer
- Journal Impact Factor
- Conferences
- Big Data
- Cloud Computing
- Human Behavior
- Structural Equation Modelling
- SEM Analysis
- Applied Mathematics
- Dynamical Systems
- Statistics
- Blockchain
- Testing
- Publications
- Amos
- EViews
- NS2
- NS3
- Data Analysis Tool
- Conceptual Framework
- CST Software
- Dissertation
- Indroduction
- Structural Analysis
- Renewable Energy
- Medicine
- AI Model
- science experiments
- FUTURE TECH