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6 years ago in Cloning Method By Sobha

How to clone DNA using plasmid

Can I clone DNA using plasmids? If yes, how it is done? Please explain the steps in detail if possible or provide me with some useful and informative videos.

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All Answers (3 Answers In All)

By Riya N Answered 6 years ago

Yes, definitely. You can use plasmids to clone the DNA. The plasmids are introduced into bacteria through a process called transformation. Bacteria with the right plasmid are then used to make more plasmid DNA or are generated to express the gene and make protein.

By Seema Answered 6 years ago

Cloning DNA using plasmids includes 3 steps.
1. Cutting and pasting DNA – A restriction enzyme recognises a particular target sequence and cuts DNA into two halves. Several restriction enzymes generate cut ends with short and single-stranded overhangs. However, if two molecules have similar overhangs, then they can base-pair and stick together.
2. Bacterial selection and transformation – Plasmids can be induced into bacteria through a process called transformation. During transformation, the prepared bacterial cells are given a shock thereby encouraging them to take up foreign DNA. A plasmid consists of an antibiotic resistance gene that enables bacteria to survive even in the presence of a specific antibiotic. Thus, bacteria that takes the plasmid can be selected.
3. Protein production – Once the bacterial colony is founded with the right plasmid, you can grow a large culture of plasmid-bearing bacteria. Also, the bacteria serve as factories churning a huge amount of protein.

By Zeba Malik Answered 6 years ago

Watch https://www.khanacademy.org/science/high-school-biology/hs-molecular-genetics/hs-biotechnology/v/dna-cloning-and-recombinant-dna, https://www.youtube.com/watch?v=ntIWA0tYv3w

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