Ask, Learn and Accelerate in your PhD Research

Login Sign up

Post Your Answer

Aseptic plant preparing method vs sterilization procedure

5 years ago in Research Methods By Zeba Malik

Research methods

Which is the best method for plant tissue culture? Am confused among technique of preparing aseptic plants and sterilization procedure.

All Answers (3 Answers In All)

By Anurag Answered 5 years ago

I would suggest you to go with aseptic plant method. The surface sterilization of plant tissue causes deleterious effect as the sterilants are toxic chemicals. Seeds are cannot resist such deleterious effect due to the presence of seed coat. Inorder to avoid surface sterilisation of plant tissue and seeds are surface sterilised and then seeds in culture give rise to an aseptic seedlings.

By Waylon Kearney Answered 5 years ago

Go with sterilization method. This method not just involves sterilization of plant materials but also sterilization of non-living materials like glass tube, nutrient medium etc used during the experiment.

By Rani Answered 5 years ago

Go with sterilization procedure. The culture medium consisting of sugar support the growth of microorganisms. Hence if they come in contact with cellular form or in spore form, the micro-organisms grow faster than the higher plant tissue and kills the tissue. Hence, the surface of plant tissue including nutrient medium should be sterilized.

View Related Questions

How do I incorporate community involvement and principles of participatory design in my Architecture study to ensure that diverse voices play a role in shaping our cities?

asked at 29 Jan, 2024 15:58 in Research Methods By Shraddha

183 Views
0 Votes
1 Answer

Phenomenology and Ethnography

asked at 16 Aug, 2021 11:16 in Research Methods By Heena

229718 Views
0 Votes
3 Answer

Which Invetigation method can help better in carrying out research for thermal management?

asked at 02 Dec, 2020 16:53 in Research Methods By Krupa

169113 Views
0 Votes
1 Answer

Fluid Mechanics

asked at 26 Nov, 2020 06:17 in Research Methods By Vishwas Rao

202627 Views
0 Votes
1 Answer

How to write an analytical research paper?

asked at 06 Aug, 2020 14:05 in Research Methods By Nidhi S

271168 Views
42 Votes
4 Answer

Drag And Drop in selenium

asked at 05 Mar, 2020 06:52 in Research Methods By Ritesh Tomar

284739 Views
47 Votes
3 Answer

Can anyone name a few new interesting biotechnology research topics/questions?

asked at 24 Jan, 2020 11:25 in Research Methods By Adi

614 Views
0 Votes
2 Answer

Difference between the literature review, theoretical & conceptual analysis

asked at 10 Jan, 2020 09:00 in Research Methods By Hema

937593 Views
144 Votes
47 Answer

Which appropriate models must use in Study "Factors Influencing adopters, Trust in Cloud computing?"

asked at 10 Jan, 2020 06:00 in Research Methods By Anu

583 Views
8 Votes
3 Answer

Research hypothesis

asked at 16 Dec, 2019 07:14 in Research Methods By Jyotsnamayee Nayak

827 Views
46 Votes
3 Answer

Can grounded theory be considered as a research design?

asked at 12 Nov, 2019 06:07 in Research Methods By Shanti

596 Views
0 Votes
3 Answer

R language codes to run the exploratory research

asked at 10 Oct, 2019 02:52 in Research Methods By Shraddha

487 Views
0 Votes
3 Answer

Types of mixed method in research

asked at 05 Oct, 2019 16:07 in Research Methods By Nazia

151722 Views
0 Votes
3 Answer

Phenomenology vs ethnography

asked at 29 Sep, 2019 17:24 in Research Methods By Sarita

715 Views
0 Votes
3 Answer

How does artificial neural network work?

asked at 28 Sep, 2019 17:49 in Research Methods By Jayalakshmi

513 Views
0 Votes
2 Answer