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Brightfield or electron microscopy technique

5 years ago in Research Methods By Priyanshu Rathore

Research objective

To gain information about microscopic specimens which technique should I use? Brightfield or electron microscopy technique?

All Answers (4 Answers In All)

By Fathima M Answered 5 years ago

Priyanshu, use brightfield technique. This is traditional microscopy, and here the cells are illuminated by visible light. This microscopy provides a general picture of cell function but the drawback is it is not very detailed or specific. The advantage of using this technique is, it uses special techniques such as phase contrast to show cellular structures in more detail especially for viewing details of animal cells as they lack cell walls. Brightfield microscopy also allows imaging of live or fixed cells and tissues.

By Deepa S Answered 5 years ago

Both brightfield and electron microscopy are equally good. I would suggest you to use electron microscopy. This technique replaces visible light instead of focused beam. Electron microscopy permits a higher magnification of specimens and is useful in obtaining detailed information of subcellular structures.

By Carol Grace Answered 5 years ago

Priyanshu use fluorescence microscopy. This uses fluorescent substances to indicate structures in a specimen. Most fluorescent substances give off visible light after excitation by UV light. For eg, DAPI is a dye which binds onto DNA. The DNA & nuclei of cells stained with DAPI emit blue light under UV light.

By Tarushi Yadav Answered 5 years ago

Priyanshu, go through various techniques and then finalise the technique. Don’t just stick onto brightfield or electron microscopy technique.a

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