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How to construct plasmid map?

5 years ago in PhD Concept paper By Simanghi

Design Discussion

Hello guys! I want to construct plasmid map using gel electrophoresis. How do I do it?

All Answers (2 Answers In All) Post Your Answer

By Trisha Answered 5 years ago

Gel electrophoresis includes following stages. 1. Retrieving digestion reaction from thaw and freezer. Then move all the contents to the bottom of the tube. 2. Pre run the E-gels with the comb for two minutes and remove the comb. 3. Dilute the samples and load the gel. 4. If a gel that was cast is being used then add ethidium bromide stock. Run the gel for 10-15 minutes and record the results.

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