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7 years ago in Research Writing By Deep

How to over come optical resolution limit?

How to over come optical resolution limit?Hello all! I’m conducting research in biophysics. I have a doubt regarding single molecule technique. How can you overcome optical resolution limit in single-molecule technique?

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By Pritam Kapoor Answered 7 years ago

Deep, to overcome optical resolution limit we use localization microscopy method (one among single-molecule technique). Optical microscopy is an appealing biophysical tool used to study small molecules. This is because it is less perturbing to their natural state when compared to other methods.

By Nithin Rao Answered 7 years ago

I agree with preetham. However, the wave nature of light limits the beam of a diameter that can be focussed to half a wavelength commonly known as the diffraction limit. But some biological molecules of interest are somewhere approximately two orders of magnitude smaller and hence we cannot study them by looking down the microscope.

By Deep Answered 7 years ago

What happens when two light emitting molecules are within a few nm?

By Dipanjan Chattopadhyay Answered 7 years ago

Deep, if this is the condition then each other their diffraction-limited images overlap and their positions cannot be determined accurately .

By Deep Answered 7 years ago

Any example that proves localization microscopy principle?

By Dipanjan Chattopadhyay Answered 7 years ago

https://arxiv.org/pdf/1704.06837.pdf.

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