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6 years ago in Research Writing By Deeksha S

Methods to analyse the proteins

Other than titration and neutralisation techniques, are there any other methods to analyse the proteins?

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By Shilpa Answered 6 years ago

Yes, there is. I know a method called protein separation method that can be used to analyse the proteins. Separation method itself consists of methods like, SDS page, isoelectric focusing, chromatic methods, and two-dimensional gel electrophoresis.

By Dev Raj Acharya Answered 6 years ago

The most common method is western blotting method. This method can be used to detect specific proteins in a given sample of extract. Here the sample of proteins is electrophoresed by SDS-PAGE and are then transferred to a membrane where they will be probed using few antibodies that are specific to the target protein.

By Deeksha S Answered 6 years ago

Thank you so much for the answers. Is there any other method?

By Ritesh Tomar Answered 6 years ago

Oh yes. There is a technique called protein identification. Read this http://njms.rutgers.edu/gsbs/olc/core1/prot/2008/Protein%20Analysis%20Methods%20evening%20core2008.pdf

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