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6 years ago in Research Methods By Varun
Steps involved in PCR
Please explain the steps involved in PCR? Can we use gel electrophoresis to predict the results of PCR?
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By Yashti Iyer Answered 6 years ago
The basic steps involved in PCR are:
Denaturation: Here heat the reaction to denature the DNA strands. This will provide you with single-stranded template for the following step.
Annealing: This step involves cooling the reaction so that the primers can easily bind to complementary sequences on the single-stranded template DNA.
Extension: In this step, you have raise the reaction temperature so the taq polymerase extends the primers, thereby synthesizing latest strands of DNA.
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By Zeba Malik Answered 6 years ago
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By Morgan Wurst Answered 6 years ago
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