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Steps involved in PCR

5 years ago in Research Methods By Varun

Research Objectives Discussion

Please explain the steps involved in PCR? Can we use gel electrophoresis to predict the results of PCR?

All Answers (5 Answers In All)

By Yashti Iyer Answered 5 years ago

Hey, The basic steps involved in PCR are: Denaturation: Here heat the reaction to denature the DNA strands. This will provide you with single-stranded template for the following step. Annealing: This step involves cooling the reaction so that the primers can easily bind to complementary sequences on the single-stranded template DNA. Extension: In this step, you have raise the reaction temperature so the taq polymerase extends the primers, thereby synthesizing latest strands of DNA.

By Zeba Malik Answered 5 years ago

Varun, you can definitely use gel electrophoresis to visualise the results of PCR. basically a gel electrophoresis is a technique where the fragments of DNA are drawn through a gel matrix and thereby the DNA fragments are separated as per the size of the fragments.

By Morgan Wurst Answered 5 years ago

To know about the steps included in the PCR process in detail, visit https://study.com/academy/lesson/pcr-synthesizing-dna-using-polymerase-chain-reaction.html, https://www.onlinebiologynotes.com/polymerase-chain-reaction-pcr-principle-procedure-steps-types-application/

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