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Best method to amplify piece of DNA

6 years ago in Case Study By Manoj

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By Meera Answered 5 years ago

The key difference include 1.Type of polymerase: In DNA replication there are many DNA polymerases but in PCR, thermostable DNA polymerases are derived from bacteria or archaea. 2.Length of DNA: In PCR the length of the DNA used is much smaller when compared to that of replication method. 3.Features of polymerase used: High fidelity, speed, and repair are the features required for DNA replication. PCR reactions utilises simpler polymerases that doesn’t have rich feature.

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