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Throughput DNA sequencing vs Sanger sequencing method

5 years ago in Case Study By Deepa S

Abstract

Why use high throughput DNA sequencing method rather than sanger sequencing approach? Is there any limitation?

All Answers (3 Answers In All)

By Natasha Answered 5 years ago

Although sanger sequencing method is used to determine the sequencing of long stretches, it cannot be used when a large number of molecules are to be sequenced. That is when high throughput DNA sequencing is used.

By Joshna Answered 5 years ago

I don’t think there is any limitation associated with sanger sequencing method. High throughput sequencing is used to understand single-nucleotide polymorphism whereas Sanger sequencing is used for denovo initial sequencing.

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