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Determination of homogenous proteins

5 years ago in Case Study By Seema


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By Shubham Answered 5 years ago

For determination of size homogeneity you can use gel filtration. Gel filtration let’s you detect small changes in the  size of detergent-protein complexes especially when different detergent-proteins are compared.  Gel filtration is used to give an indication of the suitability of various detergents for a particular protein.  Therefore separation of gel filtration is an important tool for qualifying the membrane protein preparation.


By Vishal Answered 5 years ago

 if you have 100% amino acid sequence identity then the proteins can be said to be homogenous  


By Pragati Answered 5 years ago

 Two proteins can be identical only when the structure, function and their sequences are similar.  


By Meera Answered 5 years ago

 Read https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-8-222.  


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