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Can RNA be stored at -20 degree C?
8 years ago in Case Study By Trisha
All Answers (4 Answers In All)
By Shubham Answered 5 years ago
No, RNA is not stable at -20 degree celsius. For it to be stable you have to store it at -80 to 82 degree C.
By Suma Answered 5 years ago
Well you can use DEPC treated water to dissolve RNA and store it in -80C. But I think it is always better to generate the cDNA within a day and store it in -20C. cDNA would be more stable for storage that RNA.
By Shraddha Answered 5 years ago
I had dissolved RNA in purified RNAse free water and stored it at -80C. However, cDNA is more stable and can be stored at -20C. But normally we don’t transcribe the RNA in cDNA.
By Natasha Answered 5 years ago
Refer https://www.thermofisher.com/in/en/home/references/ambion-tech-support/nuclease-enzymes/general-articles/working-with-rna.html.
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