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10 years ago in Case Study By Trisha

Can RNA be stored at -20 degree C?

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By Shubham Answered 7 years ago

No, RNA is not stable at -20 degree celsius. For it to be stable you have to store it at -80 to 82 degree C.

By Suma Answered 7 years ago

Well you can use DEPC treated water to dissolve RNA and store it in -80C.
But I think it is always better to generate the cDNA within a day and store it in -20C.
cDNA would be more stable for storage that RNA.

By Shraddha Answered 7 years ago

I had dissolved RNA in purified RNAse free water and stored it at -80C.
However, cDNA is more stable and can be stored at -20C.
But normally we don’t transcribe the RNA in cDNA.

By Natasha Answered 7 years ago

Refer https://www.thermofisher.com/in/en/home/references/ambion-tech-support/nuclease-enzymes/general-articles/working-with-rna.html.

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