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Working of confocal microscopy

5 years ago in Case Study By Vishal

Action Research

All Answers (3 Answers In All)

By Trisha Answered 5 years ago

Confocal microscopy is similar to widefield microscopy. The confocal microscope makes use of fluorescence optics. Here instead of illuminating the entire sample at a time, laser light will be focused onto a defined spot at a depth. This results in the emission of fluorescent light at a focused point. A pinhole present inside the optical pathway cuts the signals which are not in focus and allows signals that are fluorescence.

By Meera Answered 5 years ago

Yes you can. By scanning the specimen (in raster pattern only), images of a single optical plane will be created. 3D objects can then be visualised by scanning several optical planes. This is achieved by stacking them using a suitable microscopy (z-stack). It is also analyses multicolor immunofluorescence stainings by making use of state-of-the-art confocal microscopes.

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